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Table 1 Primers and probes for the qPCR method for three vertebrate host species

From: Probe-based multiplex qPCR identifies blood-meal hosts in Anopheles mosquitoes from Papua New Guinea

Organism

Primer/probe name

Nucleotide sequence (5′-3′)

Amplicon size (bp)

Human

humanTH1_F

GGCCTGTTCCTCCCTTATTT

101a

humanTH1_R

TACACAGGGCTTCCGAGT

humanTH1_P

FAM-ATGGAGTCTGTGTTCCCTGTGACC-QSY

Pig

pigCOX1_F

CTGACTACTTCCACCATCCTTC

108

pigCOX1_R

TGGGCTAAGTTTCCAGCTAAA

pigCOX1_P

VIC-ATAGTAGAAGCCGGAGCGGGTACT-QSY

Dog

dogCYTB_F

TGGACAAAGCAACCCTAACA

103

dogCYTB_R

CCGGTTTCGTGTAGAAATAGGA

dogCYTB_P

ABY-TCATCCTCCCTTTCATCATCGCAGC-QSY

  1. aDue to presence of a microsatellite repeat sequence (5′-AATG-3′) within the human amplicon, the PCR product size varies between different human individuals. Presence of the repeat sequence does not affect PCR amplification as the primers and probe sequence do not extend into the repeat region