Table 2 Analytical sensitivity and corresponding threshold cycle (Cq) values from singleplex and multiplex real-time PCR assays for each target

Canis familiaris

10⁸

17.92 ± 0.17

17.91 ± 0.15

10⁷

21.14 ± 0.06

20.38 ± 0.99

10⁶

24.43 ± 0.04

23.80 ± 0.04

10⁵

27.79 ± 0.10

27.09 ± 0.20

10⁴

31.09 ± 0.11

30.14 ± 0.19

10³

34.21 ± 0.40

33.14 ± 1.03

Homo sapiens

10⁸

17.81 ± 0.05

16.11 ± 0.31

10⁷

20.84 ± 0.12

18.34 ± 0.14

10⁶

24.12 ± 0.04

21.73 ± 0.58

10⁵

27.43 ± 0.08

25.31 ± 0.23

10⁴

30.55 ± 0.24

27.90 ± 0.37

10³

33.20 ± 0.07

30.59 ± 0.28

Leishmania spp.

10⁸

8.64 ± 0.05

9.18 ± 0.31

10⁷

12.38 ± 0.10

11.43 ± 0.14

10⁶

15.51 ± 0.12

14.61 ± 0.06

10⁵

19.11 ± 0.04

17.88 ± 0.16

10⁴

22.63 ± 0.07

21.45 ± 0.17

10³

26.20 ± 0.02

24.44 ± 0.23

10²

29.77 ± 0.13

27.46 ± 0.05

10¹

32.33 ± 0.23

30.88 ± 0.25

10⁰

33.49 ± 0.09

33.11 ± 0.25