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Table 3 Intra-assay reproducibility of the multiplex real-time PCR assay

From: Fast multiplex real-time PCR assay for simultaneous detection of dog and human blood and Leishmania parasites in sand flies

DNA sample

Quantity (fg/reaction)

Cq value

Mean ± SD

%CV

R1

R2

R3

Canis familiaris

108

17.27

17.27

17.01

17.18 ± 0.15

0.87

107

19.23

19.17

19.30

19.24 ± 0.06

0.33

106

22.98

22.22

21.73

22.31 ± 0.63

2.82

105

25.06

25.44

26.14

25.55 ± 0.55

2.15

104

28.94

29.02

28.74

28.90 ± 0.15

0.51

103

33.61

32.99

34.48

33.69 ± 0.75

2.22

Homo sapiens

108

16.67

16.99

16.87

16.84 ± 0.16

0.96

107

18.76

18.74

18.87

18.79 ± 0.07

0.39

106

22.50

22.35

22.74

22.53 ± 0.20

0.88

105

25.87

25.57

25.72

25.72 ± 0.15

0.58

104

28.48

28.52

27.83

28.27 ± 0.38

1.36

103

30.94

33.24

33.13

32.44 ± 1.30

4.01

Leishmania spp.

108

5.96

5.92

6.06

5.98 ± 0.07

1.23

107

8.58

8.87

9.16

8.87 ± 0.29

3.28

106

12.54

12.22

12.56

12.44 ± 0.19

1.53

105

15.81

15.58

16.32

15.91 ± 0.38

2.39

104

19.31

19.43

19.78

19.51 ± 0.25

1.26

103

23.16

23.06

22.72

22.98 ± 0.23

1.02

102

26.66

26.21

26.78

26.55 ± 0.30

1.13

101

29.82

29.91

29.83

29.85 ± 0.05

0.16

100

32.03

32.10

32.52

32.22 ± 0.26

0.81

  1. Abbreviations: Cq, quantification cycle; R, replicate; SD, standard deviation; %CV, percent coefficient of variation