A new piroplasmid species infecting dogs: morphological and molecular characterization and pathogeny of Babesia negevi n. sp.

Table 2 Target genes and primers used for PCR to detect Babesia spp., Borrelia persica, Hepatozoon canis, Ehrlichia canis and Ornithodoros tholozani in this study

Target organism and gene	Primer	Primer sequence (5′-3′)	Reference
Babesia 18S rRNA	PiroplasmidF	CCAGCAGCCGCGGTAATT	[11]
	PiroplasmidR	CTTTCGCAGTAGTTYGTCTTTAACAAATCT	[11]
	522F	GTTGATCCTGCCAGTAGT	[12]
	1661R	AACCTTGTTACGACTTCT	[12]
	Fragment1F	GTTGATCCTGCCAGTAGT	This study
	Fragment1R	CTGGAAAAAGAGAGCCGA
	Fragment2F	TGTAATTGGAATGATGGGAATC
	Fragment2R	AACCTTGTTACGACTTCTC
	AdditionalF	TTCCGTTAACGAACGAGACC
	AdditionalR	TTATAGTTAGGACTACGACGG
Babesia cox1	COX1F	GGAAGTGGWACWGGWTGGAC	[13]
Babesia cox1	COX1R	TTCGGTATTGCATGCCTTG	[13]
Borrelia spp. flab	Bfpbu	GCT GAA GAG CTTGGAATGCAACC	[14]
Borrelia spp. flab	Bfpcr	TGATCAGTTATCATTCTAATAGCA	[14]
Hepatozoon spp.	Hepatozoon 18S-F	GGTAATTCTAGAGCTAATACATGAGC	[16]
Hepatozoon spp.	Hepatozoon 18S-R	ACAATAAAGTAAAAAACAYTTCAAAG	[16]
Ehrlichia spp. 16S rRNA	EHR16SD	GGTACCYACAGAAGAAGTCC	[15]
Ehrlichia spp. 16S rRNA	EHR16SR	TAGCACTCATCGTTTACAGC	[15]
Tick 16S rRNA	16S+1	CTGCTCAATGATTTTTTAAATTGCTGTGG	[17]
Tick 16S rRNA	16S−1	CCGGTCTGAACTCAGATCAAGT	[17]

ISSN: 1756-3305