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Fig. 3 | Parasites & Vectors

Fig. 3

From: Genetic validation of Leishmania genes essential for amastigote survival in vivo using N-myristoyltransferase as a model

Fig. 3

Promastigote flow cytometric assay. a Flow cytometric characteristics of single and double knockouts only transfected with a single plasmid in presence and absence of ganciclovir (GCV). b In the NMT double knockout parasites the plasmid pXNG4-SAT-NMT could not be lost in the absence of a second ectopic NMT allele. c The plasmid pXNG4-SAT-NMT was lost in the NMT double knockout parasites over several passages in the presence of a second ectopic NMT allele. Mutants were kept for 2 and/or 5 passages in medium containing GCV or in medium containing either hygromycin and G418 or hygromycin, puromycin and nourseothricin, respectively, at which point they where analyzed by flow cytometry. NMTHYG/+ [tdtom] = LV9 NMTHYG/+ [ tdtom] cl. 13; NMTHYG/PAC [NMT-TK] = LV9 NMTHYG/PAC [NMT-TK] cl. 12

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