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Table 1 Overview of plasmids used and their characteristics

From: Genetic validation of Leishmania genes essential for amastigote survival in vivo using N-myristoyltransferase as a model

Abbreviation used in manuscript

Fluorescence

Antibiotic gene

N-myristoyl-transferase present?

Thymidine kinase present?

Antibiotic used for selection

NMT-TK

GFP

Neomycin phospho-transferase

Yes

Yes

G418

NMT-tdtom

tdtom

Streptothricin acetyl-transferase

Yes

No

Nourseothricin

tdtom

tdtom

Streptothricin acetyl-transferase

No

No

Nourseothricin