Table 3 Leishmania donovani mutant lines used for the qualitative and quantitative plasmid retention experiments in Figs. 5, 6, 7 and the applied selection conditions
Leishmania mutant line | Antibiotic used to select for specific plasmid(s) | ||
|---|---|---|---|
nourseothricin | G418 | nourseothricin/G418 | |
LV9 NMTHYG/+ [NMT-TK] | NMT-TK | na | na |
LV9 NMTHYG/PAC [NMT–TK] | NMT-TK | na | na |
LV9 NMTHYG/PAC [NMT–TK] [tdtom] | NMT-TK | tdtom | NMT-TK; tdtom |
LV9 NMTHYG/PAC [NMT–TK] [NMT-tdtom] | NMT-TK | NMT-tdtom | NMT-TK; NMT-tdtom |