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Table 4 Average median fluorescence intensity (MFI) values of the GFP signal in the tdtom+ gate

From: Genetic validation of Leishmania genes essential for amastigote survival in vivo using N-myristoyltransferase as a model

Selection conditionaGCVNMT HYG/PAC
[NMT-TK] [tdtom]
NMT HYG/PAC[NMT-TK] [NMT-tdtom]wt
Entryb 5.64.8 
HP3.63.2 
+4.72.6 
HPS3.64.0 
+4.84.4 
HPN4.33.0 
+5.32.8 
HPNS5.04.4 
+6.24.0 
na  2.0
+  2.1
  1. aAntibiotic combinations used for group selection (H – Hygromycin, P – Puromycin, S – Nourseothricin, N – G418)
  2. bMFI values of parasites at day of injection
  3. Abbreviation: na, not applicable