Genetic validation of Leishmania genes essential for amastigote survival in vivo using N-myristoyltransferase as a model

Table 4 Average median fluorescence intensity (MFI) values of the GFP signal in the tdtom+ gate

Selection condition^a	GCV	NMT ^HYG/PAC [NMT-TK] [tdtom]	NMT ^HYG/PAC[NMT-TK] [NMT-tdtom]	wt
Entry^b		5.6	4.8
HP	−	3.6	3.2
HP	+	4.7	2.6
HPS	−	3.6	4.0
HPS	+	4.8	4.4
HPN	−	4.3	3.0
HPN	+	5.3	2.8
HPNS	−	5.0	4.4
HPNS	+	6.2	4.0
na	−			2.0
na	+			2.1

^aAntibiotic combinations used for group selection (H – Hygromycin, P – Puromycin, S – Nourseothricin, N – G418)
^bMFI values of parasites at day of injection
Abbreviation: na, not applicable

ISSN: 1756-3305