Fig. 3

Western blot detection of anti-H3K27me3. a, b Histones of male and female parasites treated with vehicle (0.1% DMSO), 7.5 µM or 20 µM GSK-J4 for 24 h. c Nuclear protein extract of schistosomula treated with vehicle (0.1% DMSO) or with 7.5 μM GSK-J4 for 72 h were submitted to western blotting. Protein extracts were separated by 15% SDS-PAGE, transferred onto a PVDF membrane and submitted to western blotting using monoclonal anti-H3K27me3 (mAbcam 6002) at 1:1000 and anti-H3 core histone (mAbcam 24834) at 1:1000. Densitometry of autoradiograms was performed by calculating the normalized ratio of H3K27me3/H3 core histone signals. Autoradiogram bands were measured by densitometric analysis using ImageJ software (http://rsweb.nih.gov/ij/). Data are representative from one assay