Fig. 2From: One-step RT-qPCR assay for ZIKV RNA detection in Aedes aegypti samples: a protocol to study infection and gene expression during ZIKV infectionZIKV strains detected from infected Vero cells. Total RNA from Vero cells infected with ZIKV MR766 and Puerto Rico strains was used as template for one-step RT-qPCR reaction, using NS5-2362F and NS5-2457R primers. Each sample was tested in duplicateBack to article page