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Fig. 3 | Parasites & Vectors

Fig. 3

From: One-step RT-qPCR assay for ZIKV RNA detection in Aedes aegypti samples: a protocol to study infection and gene expression during ZIKV infection

Fig. 3

Alignment of the target amplicon amplified by ZIKV 1086 and ZIK 1162c primers with Aedes aegypti mRNA sequences. The nucleotides in bold on Ae. aegypti sequences indicate identities with consensus sequence on the top (Zika virus - PRVABC59 strain: polyprotein). Nucleotides highlighted in yellow correspond to primer (ZIKV 1086 and ZIK 1162c) annealing sites. Sequence highlighted in green correspond to 1107-FAM probe binding site

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