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Fig. 4 | Parasites & Vectors

Fig. 4

From: One-step RT-qPCR assay for ZIKV RNA detection in Aedes aegypti samples: a protocol to study infection and gene expression during ZIKV infection

Fig. 4

Limit of detection and efficiency of the one-step RT-qPCR assay for ZIKV RNA detection. RNAs transcribed in vitro, containing sequences of NS5 and Env genes from MR766 and PRVABC59 strains, were used as templates for RT-qPCR reactions using hydrolysis probe (1107-FAM) and SYBR Green (NS5-2362F and NS5-2457R primers) as fluorescent dyes. Cq values (mean ± standard deviation) were obtained from two technical replicates, performed in triplicate each. Coefficient of determination (R2) was calculated using GraphPad Prism 8.3.0 software

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