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Fig. 6 | Parasites & Vectors

Fig. 6

From: One-step RT-qPCR assay for ZIKV RNA detection in Aedes aegypti samples: a protocol to study infection and gene expression during ZIKV infection

Fig. 6

Detection of ZIKV from infected blood-fed (IBF) mosquitoes. Total RNA from ZIKV-infected (PRVABC59 strain) whole mosquitoes were extracted 7 days post-infection, and used as template for RT-qPCR reactions, using NS5-2362F/NS5-2457R primers and 1107-FAM probe. The absolute quantification of ZIKV RNA in mosquito samples was obtained using a standard curve constructed from in vitro transcribed RNA

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