Table 2 Primers specificity evaluation in mosquito samples

Uninfected mosquitoes

Positive/tested

0/4

2/4

0/4

Quantification cycle (Cq) value

–

30–31

–

Melting temperature

–

78 °C

–

Infected mosquitoes

Positive/tested

4/4

4/4

4/4

Quantification cycle (Cq) value

22–23

20–21

19–20

Melting temperature

–

79 °C

79.5–80 °C

Positive control

Positive/tested

4/4

4/4

4/4

Quantification cycle (Cq) value

20

17

18–19

Melting temperature

–

79 °C

80 °C