Fig. 5

Yolk deposition into oocytes and egg laying by Ae. aegypti in response to blood proteins from different vertebrates. a Mean yolk length ± SD 48 h after feeding to repletion on blood-derived proteins in PBS-containing ATP from different vertebrates. Each protein was fed to females at 200 mg per ml with whole blood from a rat serving as the positive control and PBS containing ATP serving as the negative control. Small case letters indicate treatments significantly differed from one another after a Kruskal-Wallis and post-hoc Dunn’s test (p ≤ 0.05). b Mean yolk length ± SD 48 h after feeding to repletion on cow or human hemoglobin with no additional amino acid (AA) or with addition of isoleucine (Ile), leucine (Leu), arginine (Arg), methionine (Met) or tryptophan (Trp). Within each graph different small case letters indicate treatments significantly differed from one another after a Kruskal-Wallis and post-hoc Dunn’s test (p ≤ 0.05). c Eggs laid per female after feeding to repletion on whole blood, plasma, blood cells or blood cells plus isoleucine from cow or sheep. Numbers above each treatment indicate the total number of replete females. Horizontal bars indicate the mean ± SD. Within each graph different small case letters indicate treatments significantly differed from one another after a Kruskal-Wallis and post-hoc Dunn’s test (p ≤ 0.05)