Whole blood and blood components from vertebrates differentially affect egg formation in three species of anautogenous mosquitoes

Table 1 Feeding, oviposition time and eggs produced per Ae. aegypti female provided a live rat or bottled bloods in membrane feeders

	Live rat	Bottled rat blood with anticoagulant
	Live rat	Defibrinated	Na citrate	Na heparin	EDTA
Proportion of replete females¹	46/100	67/100	66/100	61/100	59/100
Ingested blood (mg) per female	2.90±0.94^a	2.45±0.32^a	2.27±0.33^a	2.15±0.64^a	1.26±0.55^b
Females examined for time to oviposition and eggs laid²	20	8	20	18	10
Time to oviposition (days)³	3.90 ± 0.85^a	3.50 ± 1.41^a, b	3.60 ± 0.50^a,b	3.28 ± 0.9^a,b	3.00 ± 0.00^b
Total eggs per female	123.2 ± 48.0	140.6 ± 37.7	110.0 ± 44.7	115.3 ± 48.4	137.4 ± 25.1
Eggs per mg blood consumed³	46.7 ± 21.0^a	57.9 ± 15.5^a, b	49.2 ± 20.0^a	57.6 ± 26.1^a,b	69.0 ± 12.6^b

¹Females were fed in cohorts of 10 per cage for a total of 100 females per treatment. χ² = 11.76, df= 4, p= 0.02
²A subsample of replete females was examined to determine time to oviposition, total eggs per female (clutch size) and eggs per mg of blood consumed
³For each indicated row, the mean ± SD for each treatment is indicated. Different small case letters after a given mean indicates treatments significantly differed from one another after ANOVA or Kruskal-Wallis and post hoc Tukey Kramer or Dunn’s tests, respectively
Different letters to the right of means in each row indicated the treatments significantly differed from one another (p ≤ 0.05).

ISSN: 1756-3305