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Fig. 4 | Parasites & Vectors

Fig. 4

From: Molecular characterization and expression analysis of annexin B3 and B38 as secretory proteins in Echinococcus granulosus

Fig. 4

Purification and western blotting of recombinant EgAnxB3 (rEgAnxB3) (a) and rEgAnxB38 (b). Lane M Protein marker, lane 1 rEgAnxB3/rEgAnxB38 crude protein, lane 2 purified rEgAnxB3/rEgAnxB38, lane 3 pET32a(+) induced by isopropyl β-D-1-thiogalactopyranoside, lane 4 rEgAnxB3/rEgAnxB38 incubated with rabbit anti-rEgAnxB3/rEgAnxB38-immunoglobulin G (IgG), lane 5 rEgAnxB3/rEgAnxB38 incubated with negative rabbit serum, lane 6 rEgAnxB3/rEgAnxB38 incubated with serum from a sheep infected with E. granulosus, lane 7 rEgAnxB3/rEgAnxB38 incubated with serum of a healthy sheep, lane 8 crude protein of protoscoleces (PSCs) incubated with rabbit anti-rEgAnxB3/rEgAnxB38-IgG, lane 9 crude protein of PSCs incubated with negative rabbit serum IgG. For other abbreviations, see Fig. 1

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Parasites & Vectors

ISSN: 1756-3305

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