Fig. 3.
From: Molecular characterization and serodiagnostic potential of Echinococcus granulosus hexokinase
Expression, purification and western blot analysis of rEgHK. Lanes: M Molecular marker (in KDa), 1 induced E. coli BL21 (DE3) transformed with pET30a, 2 soluble supernatant fraction of induced E. coli BL21 (DE3) transformed with pET30a-EgHK, 3 pellet fraction of induced E. coli BL21 (DE3) transformed with pET30a-EgHK, 4 purified rEgHK, 5 purified rEgHK probed with anti-rEgHK sera, 6 purified rEgHK probed with pre-immunized sera, 7 purified rEgHK probed with sera from patient with CE, 8 purified rEgHK probed with sera from healthy subjects