Table 1 Primer sequences used for the quantitative real-time polymerase chain reaction
Gene | Primer sequence (5′ → 3′) |
|---|---|
GAPDH | Forward 5′-TACCCCCAATGTGTCCGTC-3′ |
Reverse 5′-AAGAGTGGGAGTTGCTGTTGAAG-3′ | |
Ciart | Forward 5′-GTTGCATCCTATGTCCGCCTGTC-3′ |
Reverse 5′-TGGCTAGTCATCTGTGGCTCTGG-3′ | |
Per2 | Forward 5′-GAAACTGAAGTCAAAACGCGTC-3′ |
Reverse 5′-GTCCCTGGTGTGGATACTATTC-3′ | |
Serpine1 | Forward 5′-ATCTTGGATGCTGAACTCATCA-3′ |
Reverse 5′-GAGAGAACTTAGGCAGGATGAG-3′ | |
Gpnmb | Forward 5′-GATCTCTATCCCTGGCAAAGAC-3′ |
Reverse 5′-CAGTTTCCTATTGGCTTGTACG-3′ | |
Ankrd1 | Forward 5′-GCTGTGAGGCTGAACCGCTATAAG-3′ |
Reverse 5′-CCAGCACAGTTCTTGACCTTGAGG-3′ | |
Cilp2 | Forward 5′-GACAAGTACGAGTACGACGTG-3 |
Reverse 5′-TTGTGTGAACGCACCATGTA-3′ | |
Tnmd | Forward 5′-GAACAGTCAGTGATTTGGGTTC-3′ |
Reverse 5′-GGTCACATTATCGCAAATCTCC-3′ | |
Col2a1 | Forward 5′-TACTGGAGTGACTGGTCCTAAG-3′ |
Reverse 5′-AACACCTTTGGGACCATCTTTT-3′ | |
Wif1 | Forward 5′-GAAATGGAGGCTTTTGTAACGA-3′ |
Reverse 5′-GCAAATACATTTTCCCGGGTAA-3′ |