Table 2 Primers of PCR and amplification conditions
From: Genetic diversity analysis of Dermacentor nuttalli within Inner Mongolia, China
Gene target | Primer sequence (5'~3') | Amplification size (bp) | Annealing temperature (°C) |
|---|---|---|---|
D16SF | ATGAAAATCTTTAAATTGCTG | 472 | 50 |
D16SR | CCTCATTCTCATCGGTCT | Â | Â |
DCO1F | CGAATAGAACTTAGCCAACCT | 1210 | 50 |
DCO1R | AATAACGACGGGGTATTCCT | Â | Â |
DITS2F | TCCGTCGACTCGTTTTGACC | 1040 | 57 |
DITS2R | GGATACATCGCTTTCGCCCAT | Â | Â |