Table 1 Primers sets for DNA amplification and sequencing of CVBD pathogens in dogs
From: Molecular survey of vector-borne diseases in two groups of domestic dogs from Lisbon, Portugal
Gene target | Pathogen | Sequence (5′-3′) | Fragment length (bp) | Reference |
|---|---|---|---|---|
16S rRNA | Anaplasmataceae | EHR16SD: GGT ACC YAC AGA AGA AGT CC EHR16SR: TAG CAC TCA TCG TTT ACA GC | 345 | Parola et al. [49] |
18S rRNA | Babesia sp. | BAB F: GTCTTGTAATTGGAATGATGG BAB R: CCAAAGACTTTGATTTCTCTC | 550 | Beck et al. [4] |
18S rDNA | Hepatozoon sp. | Hep F: ATACATGAGCAAAATCTCAAC Hep R: CTTATTATTCCATGCTGCAG | 666 | Inokuma et al. [50] |
16S rRNA | Mycoplasma sp. | Myco322s: GCCCATATTCCTACGGGAAGCAGCAGT Myco938as: CTCCACCACTTGTTCAGGTCCCCGTC | 560 | Varanat et al. [51] |