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Fig. 2 | Parasites & Vectors

Fig. 2

From: Comparative analysis of the allergenic characteristics and serodiagnostic potential of recombinant chitinase-like protein-5 and -12 from Sarcoptes scabiei

Fig. 2

Immunoblotting of IgE binding to rSsCLP5 (a) and rSsCLP12 (b). Lanes: M, protein molecular weight markers (in KDa); 1, bacteria-expressed recombinant proteins rSsCLP5 (a-1) and rSsCLP12 (b-1); 2, non-purified recombinant proteins after ultrasonication of rSsCLP5 (soluble, a-2) and rSsCLP12 (inclusion, b-2); 3, purified recombinant proteins of rSsCLP5 (a-3) and rSsCLP12 (b-3); 4–5, western blot detection with prior mite-allergic mouse sera (non-allergic; negative group); 6–7, western blot detection with mite-allergic mouse sera (experimental group); 8, western blot detection with no serum (blank control)

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Parasites & Vectors

ISSN: 1756-3305

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