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Fig. 4 | Parasites & Vectors

Fig. 4

From: MiR-4448 is involved in deltamethrin resistance by targeting CYP4H31 in Culex pipiens pallens

Fig. 4

CDC bottle assays were was used to detect the function of miR-4448 by microinjection of the miR-4448 mimic in DR strain mosquitoes. Microinjection of miR-4448 mimic could reduce mosquito resistance to deltamethrin. a, b Microinjection of the miR-4448 mimic upregulated the expression of miR-4448 (by 3.53-fold) and downregulated the expression of CYP4H31 (65.82%) in the DR strain. c Mosquito mortality was assessed after incubation for a 2 h in a CDC bottle containing 7 mg/l deltamethrin. The mortality of the miR-4448 mimic group was higher than that of the acetone control, WT, and miRNA control mimic (NC1) groups. At 105 min, in the mimic group, the mortality rate was 67.4% (31/46); in the NC1 group, the mortality rate was 43.9% (18/41); in the WT group, the mortality rate was 40.0% (24/60). At 120 min, in the mimic group, the mortality rate was 78.3% (36/46), while it was 48.8% (20/41) in the NC1 group and 46.7% (28/60) in the WT group. Data are representative of three technical replicates of three biological replicates and are indicated as the mean ± SE; ***P < 0.001, **P < 0.01, *P < 0.05. RE relative expression, DR-strain deltamethrin-resistant strain

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