Fig. 5
From: MiR-4448 is involved in deltamethrin resistance by targeting CYP4H31 in Culex pipiens pallens
CCK-8 assays were employed to detect cell viability after deltamethrin treatment. Overexpression of CYP4H31 upregulated the cell viability of C6/36 cells under deltamethrin stress. a qRT-PCR analysis of CYP4H31 in transfected C6/36 cells. The cells transfected with pIB/V5-His-CYP4H31 showed a significantly higher transcription level (851.0-fold). b Western blotting experiments using His-tag antibodies to detect the CYP4H31 protein. c The pIB/V5-His-CYP4H31 construct-transfected C6/36 cells were treated with deltamethrin and their viability was calculated using a CCK-8 kit. The viability of pIB/V5-His-CYP4H31-transfected cells was higher than that in the pIB/V5-His group. Data are representative of three technical replicates of three biological replicates and are indicated as the mean ± SE; ***P < 0.001, **P < 0.01, *P < 0.05. RE relative expression