Table 1 Oligonucleotide sequences of miR-4448, U6, CYP4H31, and β-actin used for PCR and vector constructs in the present study
From: MiR-4448 is involved in deltamethrin resistance by targeting CYP4H31 in Culex pipiens pallens
Name | Forward (5′–3′) | Reverse (5′–3′) |
|---|---|---|
pre-miR-4448 | GCTCGCACCACAACCCCG | AAGCGAGAATCATACCCCTAGACCA |
miR-4448 (RT-stem ring) | CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGCATACCCC | |
miR-4448 | ACACTCCAGCTGGGGGCTCGATGGTCTAGG | TGGTGTCGTGGAGTCG |
U6 | GCTTCGGCTGGACATATACTAAAAT | GAACGCTTCACGATTTTGCG |
CYP4H31 | ACTTTGATGGCGTTGGATAGC | AATCCCGCAAGAGGACTGAC |
β-actin | AGCGTGAACTGACGGCTCTTG | ACTCGTCGTACTCCTGCTTGG |
CYP4H31 3′ UTR-WT | CGAGCTCAAACCTGTTGATATTTTACTGGCA | CCAAGCTTTTTTTGCGCTCGATGGTTT |
CYP4H31 3′ UTR-Δ | CGAGCTCAAACCTGTTGATATTTTACTGGCA | CCAAGCTTTTTTTGCCCACCAAGGTTT |
pIB/V5-His-CYP4H31 | GGACTAGTGAGATGGAAATGCTGATTGAGATCGTACTGG | CCGCTCGAGCGGTTTCGCGCCATAATCTTCA |