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Fig. 5 | Parasites & Vectors

Fig. 5

From: Expression profiles of NOD-like receptors and regulation of NLRP3 inflammasome activation in Toxoplasma gondii-infected human small intestinal epithelial cells

Fig. 5

Caspase-cleaved ILs in response to T. gondii infection in FHs 74 Int cells. a FHs 74 Int cells were infected with T. gondii at a MOI of 10 for 0, 4, and 8 h. Protein levels of ILs were detected by western blot analysis; α-tubulin was used as the loading control. b IL-1β and IL-18 protein levels in the culture medium of FHs 74 Int cells after T. gondii infection were measured by ELISA. Graphs show quantified results in pg/ml as mean ± standard error. ***P < 0.001 compared to control. c, d FHs 74 Int cells were fixed and probed against cleaved IL-1β and IL-33. The cells were counterstained with 4′,6-diamidino-2-phenylindole (blue) and visualized using confocal microscopy. All data shown are representative of three independent experiments with similar results

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