Fig. 6

Signaling pathways involved in the regulation of NLRP3 and NLRP6 inflammasome components. a FHs 74 Int cells were infected with T. gondii at a MOI of 10 for the indicated time periods. Activation of mitogen-activated protein kinase (MAPK) subsets was evaluated by western blotting. b FHs 74 Int cells were preincubated with the 30 μM of SB203580 (p38 MAPK inhibitor) and SP600125 (JNK inhibitor), respectively, for 2 h and then infected with T. gondii at a MOI of 10 for 8 h. The indicated protein levels were then evaluated by western blot. Anti-α-tubulin was used as the internal control. Similar results were obtained in three independent experiments