Table 1 Members of the NLR family, inflammasome components and caspase-cleaved interleukins and primers used to investigate their expressions by RT-PCR and qRT-PCR
Family | Name | Synonym | Forward 5′–3’ | Reverse 5′–3’ | Amplicon length (bp) |
|---|---|---|---|---|---|
NLRC | NOD1 | CARD4 | ACTGAAAAGCAATCGGGAACT | ACACACAATCTCCGCATCTTC | 112 |
NOD2 | CARD15 | GCCTGATGTTGGTCAAGAAGA | GATCCGTGAACCTGAACTTGA | 107 | |
NLRC3 | NOD3, CLR16.2 | GGAGCCTCACCAGCTTAGATT | AGGCCACCTGGAGATAGAGAG | 117 | |
NLRC4 | IPAF, CARD12 | GGAAAGTGCAAGGCTCTGAC | TGTCTGCTTCCTGATTGTGC | 129 | |
NLRC5 | NOD27, CLR16.1 | CACCCTGACCAACATCCTAGA | TCTCTATCTGCCCACAGCCTA | 113 | |
NLRP | NLRP1 | NALP1, CARD7 | ATACGAAGCCTTTGGGGACT | ACAAAGCAGAGACCCGTGTT | 148 |
NLRP2 | NALP2 | CACCGAATGGATCTGTCTGA | GTGGTCGTTCTTTCCGTGTT | 112 | |
NLRP3 | NALP3, CIAS1 | AAAGGAAGTGGACTGCGAGA | TTCAAACGACTCCCTGGAAC | 129 | |
NLRP4 | NALP4 | CCAACGAGTTTGGCTGACTT | GCTGTCGATGACGAACAAGA | 105 | |
NLRP5 | NALP5 | CTGGGGAACGAAGGTGTAAA | GCAAGTGCAAGAAAACCACA | 122 | |
NLRP6 | NALP6 | CTGTTCTGAGCTACTGCGTGAG | AGGCTCTTCTTCTTCTTCTCCTG | 100 | |
NLRP7 | NALP7 | TAACCCGTAGCACCTGTCATC | GGTCTTCTTCCCAATGAAAGC | 101 | |
NLRP8 | NALP8 | CGCTGGTGTGCTTTCTACTTC | GGTCGGGTTTGGACATAATCT | 130 | |
NLRP9 | NALP9 | CTAGCCTCTCCCAGTCTGACAT | GCGATGTCTTCACAAACTTCAC | 121 | |
NLRP10 | NALP10 | GTCACGGTGGAGGCTCTATTT | CGAGAGTTGTCTTTCCAGTGC | 100 | |
NLRP11 | NALP11 | GTGTTGCATGTGACGTTTCC | TTTTGTTGCTCCCAATCTCC | 157 | |
NLRP12 | NALP12 | CGACCTTTACCTGACCAACAA | AGGTCCATCCCAAATAACCAG | 114 | |
NLRP13 | NALP13 | ATGGTGTGTTGGACCGTATGT | GCCAAATCTACCTCTGCTGT | 140 | |
NLRP14 | NALP14 | CCGCTTGTACTTGTCTGAAGC | GCCTCCATCTACTGGTGTGAA | 122 | |
NLRB | NAIP1 | BIRC1, NLRB1 | AGTACTTTTTCGACCACCCAGA | TAGTTGGCACCTGTGATTTGTC | 135 |
NLRA | CIITA | MHC2TA, C2TA | CCGACACAGACACCATCAAC | CCTCTGGGAAGGGTCTTTTC | 249 |
NLRX | NLRX1 | NOD9 | TGGCCTTGTCTCAGCTCTTTA | CACCAGTCCAGAACCATCTTG | 121 |
Infl. comp | Caspase 1 | IL1BC | GGGGTACAGCGTAGATGTGAA | CTTCCCGAATACCATGAGACA | 137 |
Caspase 5 | ICH-3 | TCTGTTTGCAAGATCCACGA | GTTCTATGGTGGGCATCTGG | 223 | |
Caspase 8 | ALPS2B | AGAAGAGGGTCATCCTGGGAGA | TCAGGACTTCCTTCAAGGCTGC | 142 | |
ASC | PYCARD | CTGACGGATGAGCAGTACCA | CAAGTCCTTGCAGGTCCAGT | 108 | |
Interleukins | IL-1β | IL1F2 | CCACAGACCTTCCAGGAGAA | GTGATCGTACAGGTGCATCG | 121 |
IL-18 | IL1F4 | CACCCCGGACCATATTTATT | TCATGTCCTGGGACACTTCTC | 205 | |
IL-33 | IL1F11 | GGTGACGGTGTTGATGGTAAG | CTGGCAGTGGTTTTTCACACT | 121 | |
IL-37 | IL-1F7 | CAGCCTCTGCGGAGAAAGGAAGT | GTTTCTCCTTCTTCAGCTGAAGG | 120 | |
Control | HPRT-1 | HGPRT | GACCAGTCAACAGGGGACAT | CTGCATTGTTTTGCCAGTGT | 111 |