RNA-seq analysis and gene expression dynamics in the salivary glands of the argasid tick Ornithodoros erraticus along the trophogonic cycle

Table 5 Protease inhibitors differentially upregulated 7 days after feeding (7 vs 0)

Description	Accession	No. transcripts	RPKM 0	RPKM 7	Log₂FC
Kunitz domain-containing proteins
Carboxypeptidase inhibitor SmCI-like isoform X2	XP_022249736	1	5.601	55.65	3.31
Chymotrypsin inhibitor	BAM28739, ACF57858, XP_020288964, XP_019890240	7	128.53	290.81	1.10–1.27
CLUMA_CG020329, isoform A	CRL07350	1	26.10	63.20	1.28
Dual kunitz salivary protein	ABR23474, ACB70326, ACB70328, ACB70330	24	963.30	10,876.82	1.71–5.17
Hypothetical protein B4U80_08130	RWS20793	1	1.28	4.07	1.67
Kunitz domain	ABI52641	2	14.11	45.86	1.69–1.71
Kunitz domain-containing salivary protein	ABR23431	14	1666.76	5958.42	1.70–1.94
Kunitz-type serine protease inhibitor	XP_023347625, XP_023347625, PRD26526, ODN00404	4	204.65	823.26	1.98–2.05
Papilin-like	XP_022252622, XP_023226201, XP_011307577, XP_026316323, XP_026316271	7	365.94	1573.38	2.05–2.14
Savignygrin	AAM54047, AAM54048	14	164.70	789.52	2.15–2.44
Serine protease inhibitor	B7PWG9, B7PG23, B7Q3H2	11	96.72	537.99	2.45–2.58
TIL-domain inhibitors
Chymotrypsin-elastase inhibitor	P83516	2	21.52	217.19	3.33–3.34
Ixodidin	ACB70299	4	486.49	6598.35	3.35–4.24
Riddle, putative (Fragment)	B7QLA0	3	67.50	1349.76	4.25–4.48
Trypsin inhibitor-like	XP_028178942.1	1	2.26	51.32	4.51
Other protease inhibitors
Alpha-2-macroglobulin	AAN10129, XP_023243497	14	421.09	3730.10	2.60–3.29
Cystatin	AZB52851	1	27.13	55.61	1.03
Kazal-type serine protease inhibitor domain protein	DAA34653	2	57.08	118.07	1.04–1.05
Pregnancy zone protein-like	XP_022257127	1	1.74	17.44	3.32

For each protein description, the accession codes and number of transcripts assigned, average expression level in RPKM for unfed (RPKM 0) and 7-day fed (RPMK 7) females, and the range of log₂FC reached are provided

ISSN: 1756-3305