Fig. 3

a Maximum likelihood and b neighbor-joining trees inferred from uni-, tetra- and octonucleated cyst-producing Entamoeba spp. SSU rDNA locus (542 bp, n = 106). Support for the branching order was determined b 1,000 bootstrap replicates, and only values > 70% are reported. The number of nuclei in the mature cyst are shown. BAG Bagre, CAM Cachoeiras de Macacu, SIRN Santa Isabel do Rio Negro, SJPI São João do Piauí, TER Teresina. GenBank accession numbers are indicated. Further details for reference strains can be found in Additional file 1: Table S1