Skip to main content

Table 3 Entamoeba spp. microscopy and PCR results in the present study

From: Epidemiology, species composition and genetic diversity of tetra- and octonucleated Entamoeba spp. in different Brazilian biomes

Biome/locality

Samples

Result

Microscopy-positive

N (%)

PCR-positive*

N (%)

Microscopy-positive + PCR-positive

N (%)

Microscopy-negative + PCR-positive

N (%)

Entamoeba spp. sequences obtained**

N (%)

Amazon

 Santa Isabel do Rio Negro (AM)

392

160/392 (40.8)

38/98 (38.7)

16/38 (42.1)

22/38 (57.9)

23/38 (60.5)

 Bagre (PA)

362

65/362 (17.9)

36/59 (61)

30/36 (83.3)

6/36 (16.7)

27/36 (75)

Caatinga and Cerrado

 Teresina (PI)

298

59/298 (19.7)

14/26 (53.8)

10/14 (71.4)

4/14 (28.6)

4/14 (28.5)

 São João do Piauí (PI)

131

31/131(23.6)

16/33 (48.4)

13/16 (81.3)

3/16 (18.7)

4/16 (25)

Atlantic Forest

 Cachoeiras de Macacu (RJ)

545

72/545 (13.2)

14/37 (37.8)

8/14 (57.1)

6/14 (42.9)

2/14 (14.2)

Total

1728

387/1728 (22.3)

118/253 (46.6)

77/118 (65.3)

41/118 (34.7)

60/118 (50.8)

  1. Only fecal samples positive on light microscopy were subjected to molecular analyses for species characterization and phylogenetic studies. Some Entamoeba spp. negative samples on microscopy were selected, randomly or when another individual from the same household was positive, to be tested on PCR. AM Amazonas, PA Pará, PI Piauí, RJ Rio de Janeiro. N: absolute number. (%): percentage. *Includes microscopy positive and negative results. **Sequences from other organisms were also obtained, including fungi, bacteria, plants and other intestinal protozoa (Endolimax genus)