Table 2 Primers used in quantitative PCR
From: Expression of Ascaris lumbricoides putative virulence-associated genes when infecting a human host
Genes | Sequences (5′–3′) | Product size (bp) | Tm (°C) |
|---|---|---|---|
β-actin | F: 5′-CTCGAAACAAGAATACGATG-3′ R: 5′-ACATGTGCCGTTGTATGATG-3′ | 450 | 57 |
18S | F: 5′-ATCGGTCGCGTAGGGTGGCT-3′ R: 5′-AAGCCGCAGGCTCCACTCCT-3′ | 200 | 57 |
ABA-1 | F: 5′-ACAACAAGCAACAGAAAAGC-3′ R: 5′-TGACCTCGGAAAGCATCT-3′ | 155 | 57 |
PEBP | F: 5′-CGATAGTGGCGTTGAGGT-3′ R: 5′-GGAATTTGGGGGTTTCAC-3′ | 152 | 57 |
CATH-B | F: 5′-GCTGCTGTAAAAGTTGTG-3′ R: 5′-AAGGTGGGAAAGGATAAG-3′ | 138 | 57 |
CATH-L | F: 5′-AGGCAAGGAGATGAAGTG-3′ R: 5′-GCATTGTGGCTCGTAGTA-3′ | 196 | 57 |
HSP70 | F: 5′-TACAACAAAGGCAAACTCAC-3′ R: 5′-CTCGCATTCATCCAAAAG-3′ | 171 | 57 |
AQUAPORIN | F: 5′-GGAAGAGTGAGGCGAAAT-3′ R: 5′-CCGATGAACAGAAGCAAG-3′ | 130 | 57 |
VENOM | F: 5′-TCAGAGGTGGACGACTAT-3′ R: 5′-GACAAACGACAATGATACTG-3′ | 172 | 57 |
INTEGRIN | F: 5′-ATCAACACCCGAGCAACT-3′ R: 5′-AGCCAAGCACCACTAACTG-3′ | 144 | 57 |
CADHERIN | F: 5′-GTCAGGTCTCGTCAATCG-3′ R: 5′-TGGCACTTCAACATCGTAG-3′ | 183 | 57 |
SOD | F: 5′-CCCGATTTACCATACGAC-3′ R: 5′-CCACCACCATTGAACTTC-3′ | 197 | 57 |
CLEC | F: 5′-CGGCTTTGACGAGATAGAG-3′ R: 5′-AAGACCACCGACCAGTTT-3′ | 182 | 57 |