Table 3 BLASTX and Blast2GO analyses of putative Ascaris lumbricoides virulence-associated genes
From: Expression of Ascaris lumbricoides putative virulence-associated genes when infecting a human host
Genes | Gene Ontology term analysis (Blast2Go)a | Gene description | Species | Identity (%) | E value |
|---|---|---|---|---|---|
ABA-1 | F: retinol binding | 1) ABA-1 allergen 2) ABA-1 allergen 3) Chain A, the solution structure of ABA-1A saturated with oleic acid | Ascaris lumbricoides A. lumbricoides A. suum | 100 99 99 | 6e–144 4e–56 4e–52 |
PEBP | No GO terms | 1) Phosphatidylethanolamine-binding-like protein 2) Phosphatidylethanolamine-binding-like protein 3) Phosphatidylethanolamine-binding-like protein | Toxocara canis Ancylostoma duodenale Teladorsagia circumcincta | 91 84 78 | 9e–112 2e–89 7e–88 |
CATH-B | F: cysteine-type endopeptidase activity C: extracellular space C: lysosome P: proteolysis involved in cellular protein catabolic process | 1) Cathepsin B-like cysteine proteinase 2) Cathepsin B-like cysteine proteinase 6 3) Papain family cysteine protease | A. suum T. canis Haemonchus contortus | 100 84 76 | 0.0 0.0 0.0 |
HSP70 | No GO terms | 1) Uncharacterised protein C30C11.4 2) Hypothetical protein WUBG_09411 3) Hypothetical 86.9-kDa protein C30C11.4 in chromosome III, putative | T. canis Wuchereria bancrofti Brugia malayi | 91 65 64 | 0.0 0.0 0.0 |
CATH-L | F: cysteine-type endopeptidase activity F: serine-type endopeptidase activity F: protein binding C: extracellular space C: lysosome C: vesicle lumen C: yolk granule P: proteolysis involved in cellular protein catabolic process | 1) Cathepsin L 2) Cathepsin L 3) Cathepsin L family | A. suum T. canis Caenorhabditis elegans | 100 89 82 | 2e–121 4e–107 8e–102 |
AQUAPORIN | F: channel activity C: membrane P: transmembrane transport | 1) Aquaporin-3 2) Aquaporin-1 3) Aquaporin-1 | T. canis T. canis T. canis | 94 93 93 | 5e–113 0.0 0.0 |
VENOM | C: extracellular region | 1) Venom allergen 5 2) Ancylostoma secreted protein 3) Ancylostoma secreted protein | T. canis T. canis T. canis | 58 51 50 | 6e–30 3e–65 2e–62 |
SOD | F: superoxide dismutase activity F: metal ion binding P: removal of superoxide radicals P: oxidation–reduction process | 1) Superoxide dismutase (Mn) 1, mitochondrial 2) Manganese superoxide dismutase 3) Mitochondrial manganese superoxide dismutase | T. canis Ditylenchus destructor Bursaphelenchus mucronatus | 90 75 73 | 5e–131 3e–111 6e–114 |
INTEGRIN | No GO terms | 1) Integrin alpha pat-2 2) Integrin alpha pat-2 3) Hypothetical protein WUBG_12493 | T. canis Loa loa W. bancrofti | 89 78 77 | 0.0 0.0 1e–155 |
CADHERIN | F: calcium ion binding C: plasma membrane P: homophilic cell adhesion via plasma membrane adhesion molecules C: integral component of membrane | 1) Protocadherin-16 2) Cadherin domain protein 3) Cadherin domain containing protein | T. canis Ancylostoma ceylanicum H. contortus | 77 33 40 | 9e–164 3e–11 5e–62 |
CLEC | F: transmembrane signalling receptor activity C: extracellular region C: integral component of plasma membrane F: carbohydrate binding F: monosaccharide binding | 1) C-type lectin 2) C-type lectin domain containing protein 3) C-type lectin domain family 4 member E-like | C. elegans H. contortus Pelodiscus sinensis | 26 26 33 | 1e–07 2e–06 1e–06 |