Fig. 2

Effects of the PLK inhibitor GW on flagella formation in G. lamblia. Cells were treated with 5 µM GW (closed bars) or 0.1% DMSO (open bars) for 18 h, and then stained with Giemsa solution. a Representative figures showing how to measure the cytoplasmic anterior flagella (AF), membrane-bound AF, cytoplasmic posterolateral flagella (PF), membrane-bound PF, cytoplasmic caudal flagella (CF), membrane-bound CF and membrane-bound ventral F (VF). Scale bars: 2 μm. b Flagellar length was measured in 35 cells per each condition. Data are presented as the mean of three independent experiments. Asterisks indicate that difference is statistically significant at *P < 0.01 and **P = 0.01–0.05