Table 1 Primers and morpholinos used in this study
From: A polo-like kinase modulates cytokinesis and flagella biogenesis in Giardia lamblia
Name | Nucleotide sequence (5′–3′)a, b |
|---|---|
Transgenic Giardia lamblia expressing HA-tagged GlPLK | |
 Pplk-F | CATCGCGGCCGCGTAGGCGTCATCCGAGGTGAAC |
 PLK-NL-R | GTTACGTCGACGTCTATTTGTGGATACTCGGCTT |
 Pplk-R | GTTACAAGCTTGGGGCTATAAAATTTTACAGAG |
 PLK-PBD-F | GTTACAAGCTTCCACCGTGCTACATCATGTCCTG |
 PLK-PBD-R | GTTACGTCGACTCCCCTCCCTGACCGAGCTGCCT |
Mopholino sequences | |
 Control | CCTCTTACCTCAGTTACAATTTATA |
 Anti-glplk | AGCTCCCACCGCAAAAGCCAAAATT |
Real-time PCR primers | |
 PLK-RT-F | GTCACGTTTATGAGCGAGAA |
 PLK-RT-R | CTATTCCCCTCCCTGACCGA |
 Actin-F | GTCCGTCATACCATCTGTTC |
 Actin-R | GTTTCCTCCATACCACACG |
Kinase assay | |
 PLK-GBK-F | GCACGAATTCATGTCCCACAGCAACGCCCCAGAA |
 PLK-GBK-R | CTACAGCGGCCGCCTATTCCCCTCCCTGACCGAGCT |
Mutagenesis of glplk | |
 PLKT183A-F | TGGGCCATGTGTGGAGCGCCAAACTTT |
 PLKT183A-R | GAGAAAGTTTGGCGCTCCACACAT |
Recombinant protein for antibodies | |
 rGlPLK-F | GATCGAATTCGATGTCCCACAGCAACGCCCCA |
 rGlPLK-R | CGATCTCGAGTTCCCCTCCCTGACCGAGCTG |
 rGlGAP1-F | GATTGAATTCATGCCTATTCGCCTCGGAAT |
 rGlGAP1-R | GCCTAGCGGCCGCGGCAGCCCTTGGACCCGACGTA |
 rGlCENH3-F | GATCCATATGATGAGCGGAGGCTCACGG |
 rGlCENH3-R | TACCGTCGACCCGTAGTGAATTTAAGTT |