Table 2 Strains and plasmids used in this study
From: A polo-like kinase modulates cytokinesis and flagella biogenesis in Giardia lamblia
Organism/plasmid | Description | Source/references |
|---|---|---|
Giardia lamblia | ||
 ATCC 30957 | Clinical isolate | ATCC |
Escherichia coli | ||
 DH5α | supE44, ΔlacU169 (Φ80 lacZ ΔM15), hsdR17, recA1, endA1, gyrA96, thi-1, relA1 | Invitrogen, Thermo Fisher Scientific (Carlsbad, CA, USA) |
 BL21 (DE3) | F′, ompT, hsdSB(rB−mB−) gal, dcm (DE3) | Invitrogen, Thermo Fisher Scientific |
Plasmids | ||
 pKS-3HA.neo | Shuttle vector, AmpR, neo gene | [17] |
 pGlPLK.neo | pKS_3HA.neo, 2184 bp, encoding glplk (GiardiaDB ID GL50803_104150) | This study |
 pGlPLKKDL.neo | pKS-3HA.neo, 1443 bp, encoding kinase domain and linker of glplk | This study |
 pPplk-3HA.neo | pKS-3HA.neo, 150 bp, encoding promoter region of glplk | This study |
 pGlPLKPBD.neo | pKS-3HA.neo, 894 bp, encoding promoter region and PBDs of glplk | This study |
 pGlPLKK51R.neo | pKS-3HA.neo, 2184 bp, encoding K51R glplk | This study |
 pGlPLKT179A.neo | pKS-3HA.neo, 2184 bp, encoding T179A glplk | This study |
 pGlPLKT183A.neo | pKS-3HA.neo, 2184 bp, encoding T183A glplk | This study |
 pGlPLKT179AT183A.neo | pKS-3HA.neo, 2184 bp, encoding T179AT183A glplk | This study |
 pGBKT7 | Gal4p(1–147) DNA-BD, TRP1, KanR, c-Myc Epitope | Clontech, Takara Bio (Mountainview, CA, USA) |
 pGBK-GlPLK | pGBKT7, 2037 bp, encoding glplk | This study |
 pGBK-GlPLKK51R | pGBKT7, 2037 bp, encoding K51R glplk | This study |
 pGBK-GlPLKT179A | pGBKT7, 2037 bp, encoding T179A glplk | This study |
 pGBK-GlPLKT183A | pGBKT7, 2037 bp. encoding T183A glplk | This study |
 pGBK-GlPLKT179AT183A | pGBKT7, 2037 bp, encoding T179AT183A glplk | This study |
 pET32b | Expression vector, AmpR | Novagen (Merck Biosciences, Merck AG (Darmstadt, Germany) |
 pET32-GlPLK | pET32b, 2037 bp, encoding GlPLK | This study |
 pGEX4T-1 | Expression vector, AmpR, GST | GE Healthcare (Chicago, IL, USA) |
 pGEX-GlGAP1 | pGEX4T-1, 1011 bp, encoding glgap1 | This study |
 pET21b | Expression vector, AmpR | Novagen |
 pET-GlCENH3 | pET21b, 471 bp, encoding glcenh3 | This study |