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Table 1 Primers used for rapid amplification of cDNA ends, in situ hybridization, double-stranded RNA synthesis for knockdown studies and quantitative PCR of the target gene and reference gene for Lepeophtheirus salmonis and Salmo salar

From: Roles of three putative salmon louse (Lepeophtheirus salmonis) prostaglandin E2 synthases in physiology and host–parasite interactions

Genea

Forward primer (5′-3′)

Reverse primer (5′-3′)

Useb

LsMGST1

GGAATTAATGACGATTTATGGGCTAGT

TGTCATTGAGATGCGCTCTTCTTACAC

R, IS, K

LsMGST1

TTTCGCCAATCAAGAGGACGCCAA

GGGCGCAATGAGAAGAAAGGGGACT

qPCR

LsPGES2

AAAGGTGGATCATTTGGTGGCGG

CGAGAGCTCACAGCCCATTTCATCTT

qPCR

LsPGES3L

CCAAGGTGATTTGGGCTCAAACCG

TCCTTTCCGCCCACGCCACTAA

qPCR

LsPGES3L

ATGGAGCTCTTCGACGAGGTCGTT

CCCCGCTATCAGATTGGTCCAAG

R, IS, K

LsEF1α

GGTCGACAGACGTACTGGTAAATCC

TGCGGCCTTGGTGGTGGTTC

qPCR ref

LsADT3

CTGGAGAGGGAATTTGGCTAACGTG

GACCCTGGACACCGTCAGACTTCA

qPCR ref

Ls18s

GCAGCAGGCACGCAAATT

GATGAGTCCGGCTTCGTTATTTT

qPCR ref

SsC3a

ATTCTTCCCCTCCACTCCCTCG

CGATTTGGTCGTCAAGCCAGG

qPCR

SsIL1β

GCTGGAGAGTGCTGTGGAAGA

TGCTTCCCTCCTGCTCGTAG

qPCR

SsIL4/13a

CGTACCGGCAGCATAAAAATCACCATTCC

CCTTGCATTTTGTGGTGGTCCCA

qPCR

SsIL8

GCATCAGAATGTCAGCCAGCC

ACGCCTCTCAGACTCATCCC

qPCR

SsIL10

ATGAGGCTAATGACGAGCTGGAGA

GGTGTAGAATGCCTTCGTCCAACA

qPCR

SsTNFα

CACTGCCACCAAGAGCCAAG

CGCCAGTTGTCATCGCATACC

qPCR

SsIFNγ

ATGGATGTGTTATCAAGGGCTGTGATGTG

CAGCTGGTCCTTGGAGATCTTATAGTGGAC

qPCR

SsMHC2

GGACGTGAGGTGAAGTCTGATGTGACC

CTGATGTGCTCCACCATGCAGGA

qPCR

SsCD4

GAGTACACCTGCGCTGTGGAAT

GGTTGACCTCCTGACCTACAAAGG

qPCR

SsCD8α

TAGAGTGCAAGACAACGCTGGAATGGA

TCTCGAGCCTTTTTGAAAGCCTTCAG

qPCR

SsCD8β

CAGCCTCAACAATGCTGACCGGACCTA

GCGTCCTCTGCCGTTATATTATTGATGC

qPCR

SsNCCRP1

AATCCTGCGCCTCACGGTGTGAGTC

GCGAGGAGGTCCTTCTGGTGGAAAC

qPCR

SsIgM

TGAGGAGAACTGTGGGCTACACT

TGTTAATGACCACTGAATGTGCAT

qPCR

SsIgT

GGTGGTCATGGACGTACTATTT

CCTGTGCAGGCTCATATCTT

qPCR

SsEF1α

CACCACCGGCCATCTGATCTACAA

TCAGCAGCCTCCTTCTCGAACTTC

qPCR ref

SsTRIM

TTACTGTAGGAGCTGTATTGAGGGCTGCTG

TTCTCCACCAGCTCAGCCAACATG

qPCR ref

  1. aLs, Lepeophtheirus salmonis; Ss, Salmo salar
  2. bR, RACE (rapid amplification of cDNA ends ); IS, in situ hybridization, K , knockdown; qPCR, quantitative PCR of target gene; qPCR ref., quantitative PCR of reference gene