Fig. 4

Gene expression analysis of E. granulosus (s.s.) PSC exposed to two concentrations (50 and 250 μM) of the different drugs in comparison with the control. The evaluated genes were calcineurin A, calcineurin B and calmodulin. The vertical axis of the charts represents the levels of mRNA expression (fold change: log10) in the exposed PSC and in the control group (fold change: 1). Fold change was calculated using REST 2009. The horizontal bars represent the mean ± standard deviation of three repetitions of triplicate experiments. The 2^−△△CT was the method of analysis. Asterisks indicate statistically significant differences from the control at *P < 0.05 and ****P < 0.0001, Tropi Tropisetron, Grani granisetron, CsA cyclosporine A, ABZSO Albendazole sulfoxide