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Fig. 2 | Parasites & Vectors

Fig. 2

From: Apoptosis-like cell death upon kinetoplastid induction by compounds isolated from the brown algae Dictyota spiralis

Fig. 2

A Hoechst stain assay against promastigote stage Leishmania amazonensis incubated with IC90 of compounds 13 for 24 h. Images (×40) obtained using the EVOS FL Cell Imaging System (Thermo Fisher Scientific). Overlay is represented with (a–d) and without (a′–d′) visible channel. Shown are negative control cells with DMSO (a and a′), compound 1 (b and b′), compound 2 (c and c′) and compound 3 (d and d′). B Double-stain assay against promastigote stage Leishmania amazonensis incubated with IC90 of compounds 13 for 24 h. Miltefosine is used as the positive control and DMSO as the negative control. Images (×100) were obtained using the EVOS FL Cell Imaging System with overlay of visible, blue and red channel. The presence of Hoechst stain corresponds to the presence of chromatin condensation (blue) in treated cells. Red fluorescence corresponds to the staining by propidium iodide (PI), indicating dead cells

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