Fig. 3

A Hoechst stain assay against epimastigote stage Trypanosoma cruzi incubated with IC₉₀ of compounds 1–3 for 24 h. Images (×40) obtained from the EVOS FL Cell Imaging System. Overlay is represented with (a to d) and without (a′ to d) visible channel. Shown are negative control cells with DMSO (a and a′), compound 1 (b and b′), compound 2 (c and c′) and compound 3 (d and d′). B Double-stain assay against epimastigote stage Trypanosoma cruzi incubated with IC₉₀ of compound 1–3 for 24 h. Miltefosine is used as the positive control and DMSO as the negative control. Images (×100) were obtained using the EVOS FL Cell Imaging System with overlay of visible, blue and red channel. The presence of Hoechst stain corresponds to the presence of chromatin condensation (blue) in treated cells. Red fluorescence corresponds to staining by PI, indicating dead cells