Fig. 8
a Reactive oxygen species accumulation assay. Bar graph represents means of fluorescence emission in relative fluorescence units (RFU) of L. amazonensis and T. cruzi after 24 h of incubation with the IC90 of compounds 1–3 compared to the negative control (DMSO). Miltefosine and benznidazole were used as positive controls. Differences between the values were assessed using one-way analysis of variance. Data are presented as the mean ± SD. owercase letters a–c or uppercase letters A–C indicate that means within compounds with different letters are significantly different (P < 0.05). b Images (×100) are representative of the parasites observed in the performed experiments using the EVOS FL Cell Imaging System