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Table 2 Estimates of evolutionary divergence between the internal transcribed spacer-1 rDNA region sequences of “Besnoitia-acinonyx” (MW468050; this study) and other Besnoitia spp. [B. besnoiti (AY833646), B. jellisoni (AF076860), B. neotomofelis (HQ909085), B. akodoni (AY545987), B. oryctofelisi (AY182000, GU479632), B. darlingi (AF489696, MF872605, GU479631, HQ163919], Toxoplasma gondii (L49390), Neospora caninum (AY259040), Hammondia heydorni (AY189897) and H. triffitae (KJ396594)

From: Molecular analysis suggests that Namibian cheetahs (Acinonyx jubatus) are definitive hosts of a so far undescribed Besnoitia species

Isolate

Besnoitia-acinonyx

T. gondii

N. caninum

H. heydorni

H. tiffitae

B. besnoiti

B. jellisoni

B. neotomofelis

B. akodoni

B. oryctofelisi (n = 2 sequences)

B. darlingi (n = 4 sequences)

Besnoitia-

acinonyx

0.000

          

Toxoplasma gondii

0.595

0.000

         

Neospora caninum

0.544

0.257

0.000

        

Hammondia heydorni

0.542

0.288

0.269

0.000

       

H. triffitae

0.531

0.297

0.268

0.011

0.000

      

Besnoitia besnoiti

0.234

0.530

0.492

0.534

0.523

0.000

     

B. jellisoni

0.095

0.674

0.674

0.608

0.596

0.279

0.000

    

B. neotomofelis

0.095

0.692

0.657

0.610

0.598

0.288

0.026

0.000

   

B. akodoni

0.079

0.617

0.628

0.570

0.558

0.294

0.063

0.063

0.000

  

B. oryctofelisi (n = 2 sequences)a

0.069

0.639

0.609

0.540

0.529

0.268

0.054

0.054

0.030

0.000

 

B. darlingi (n = 4 sequences)b

0.064 (0.069)

0.645 (0.654)

0.629 (0.638)

0.571 (0.578)

0.559 (0.567)

0.271 (0.277)

0.049 (0.053)

0.048 (0.053)

0.026 (0.030)

0.013 (0.017)

0.000 (0.004)

  1. The number of base substitutions per site between sequences are shown in the table (pairwise distance). Analyses were conducted using the maximum composite likelihood model [37]. This analysis involved 15 nucleotide sequences. All codon positions (1st, 2nd, 3rd, Noncoding) were included. All ambiguous positions were removed for each sequence pair (pairwise deletion option). There were 425 positions in the final dataset. Evolutionary analyses were conducted in MEGA X [36]
  2. Pairwise distances > 0.5 are underlined. Distances < 0.1 are given in italics
  3. aThe two sequences of B. oryctofelisi revealed the same values for all cells
  4. bThree of the four sequences of B. darlingi revealed the same values for all cells; the fourth sequence of B. darlingi (MF872605) revealed slightly different values which are shown in parenthesis