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Table 3 Transmission study: Detection of SARS-CoV-2 viral RNA and infectious virus in substrates, transfer containers and flies at 24 h post-transfer

From: Mechanical transmission of SARS-CoV-2 by house flies

Substrate group

PCR+, substrate/N (copy number/µl of sample)

PCR+, container surface/N (copy number/µl of sample)

PCR+, flies/N (copy number/µl of sample)

VI /IFA+, flies/N

Medium only (negative control)

0/2 (ND)

0/2 (ND)

0/10(ND)

0/10

Virus-spiked medium (positive control)

1/2 (2.5 × 101)a

2/2 (8.7 × 104)a

10/10 (6.3 × 105)a

0/10

Virus-spiked milk

2/2 (1.4 × 103)b

2/2 (1.1 × 105)b

10/10 (5.7 × 106)b

1/10

  1. N, Total number of samples (wicks, flies or swabs); ND, not detected
  2. aSD of virus-spiked medium group substrate, swabs and flies at 24 h post-transfer: 0, 1.8 × 104 and 1.7 × 106, respectively
  3. bSD of virus-spiked milk group substrate, swabs and flies at 24 h post-transfer: 1.2 × 103, 7.4 × 104 and 4.3 × 106, respectively
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Parasites & Vectors

ISSN: 1756-3305

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