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Fig. 2 | Parasites & Vectors

Fig. 2

From: CRISPR/Cas12a-based on-site diagnostics of Cryptosporidium parvum IId-subtype-family from human and cattle fecal samples

Fig. 2

Schematic of the RPA and CRISPR-Cas12a-based detection assay. a Diagram of Cryptosporidium parvum chromosome 6 showing primers, target sequence and crRNA. RPA primers are indicated by black rectangles; the PAM and target sequences are represented by red and blue rectangles, respectively. b Schematic of ReCTC-based diagnosis workflow. The RPA amplicon is used directly as the input of the ReCTC-based detection, and a ternary complex forms if the target DNA exists. F fluorophore, Q quencher, B biotin, F FAM

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