Fig. 4

Optimization of the reporter concentration for the ReCTC-based LFS detection. Various concentrations (200, 100, 50, 20, 15, 10, 5 nM) of FAM-TTATT-biotin ssDNA reporter were tested to avoid false-positive and -negative results. The concentrations used were labeled on the LFS pads, and false-positive results wwew eliminated with 20 nM FAM-TTATT-biotin ssDNA reporter or higher concentrations