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Fig. 5 | Parasites & Vectors

Fig. 5

From: CRISPR/Cas12a-based on-site diagnostics of Cryptosporidium parvum IId-subtype-family from human and cattle fecal samples

Fig. 5

Sensitivity of the ReCTC-based detection. Sensitivity test of ReCTC-based fluorescence (a) and LFS (b) assay using cloned recombinant plasmid DNA. The LOD of both the fluorescence and LFS assay was determined as 1.0 × 10–18 M cloned recombinant plasmid DNA. A1–A8: The concentrations of cloned recombinant plasmid DNA were 1.0 × 10–12, 1.0 × 10–15, 1.0 × 10–18, 1.0 × 10–19, 1.0 × 10–20, 1.0 × 10–21, 1.0 × 10–22, 1.0 × 10–23 M, respectively. Sensitivity test of ReCTC-based fluorescence (c) and LFS (d) assay using crude DNA extracted from purified oocysts. The LOD of both the fluorescence and LFS assay was determined as one and ten oocysts per milliliter, respectively. C1–C8: The numbers of oocysts per milliliter were equivalent to 1 × 105, 1 × 104, 1 × 103, 1 × 102, 1 × 101, 1, 0.1 and 0, respectively. The concentrations of cloned recombinant plasmid DNA and the numbers of oocysts per milliliter used in the sensitivity test of LFS assay (b, d) were indicated on the LFS pads

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