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Fig. 6 | Parasites & Vectors

Fig. 6

From: CRISPR/Cas12a-based on-site diagnostics of Cryptosporidium parvum IId-subtype-family from human and cattle fecal samples

Fig. 6

Specificity of the ReCTC-based detection. Recombinant pUC57 plasmid DNA containing gp60 gene of IIa, IIb, IIc, IId, IIe and IIf SFs of C. parvum (1–6) and genomic DNA of C. andersoni, C. hominis, C. meleagridis, C. muris, C. bovis, C. ryanae, Enterocytozoon bieneusi, Giardia duodenalis, Blastocystis hominis and Cyclospora cayetan (7–16) were included. a Specificity test of the ReCTC-based fluorescence detection assay. Only the sample of C. parvum IId SF exhibited a strong fluorescence signal. b Specificity test of the ReCTC-based LFS detection assay. A clear test line was observed only on the LFS where C. parvum IId SF recombinant pUC57 plasmids DNA was added

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Parasites & Vectors

ISSN: 1756-3305

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