Fig 4

Reactivity of the bovine anti-6cys A and 6cys B polyclonal antibodies against native 6cys proteins. a Immunoblot analysis of B. bovis merozoite stage (MS) and induced sexual stage (ISS) lysates. Antibodies and serum used are indicated above each lane. b Comparison of the in vitro neutralization activity of antibodies on the development of sexual stages, performed on the T2Bo B. bovis strain. The Y axis represents % of infected RBC “orange bar chart” or the % of sexual forms “blue bar chart.” The X axis represents the rabbit polyclonal antibodies against 6cys A and B peptides and sera from r6cys A and r6cys B immunized cattle. P values > 0.05 indicate no significant difference and P values < 0.05 indicates there is significant difference. The abbreviations used in the figures are explained in Additional file 1: Table S1