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Fig. 1 | Parasites & Vectors

Fig. 1

From: Transcriptomic profiling of three-dimensional cholangiocyte spheroids long term exposed to repetitive Clonorchis sinensis excretory-secretory products

Fig. 1

H69 cell spheroid formation in microwells and immunostaining of Ki67 and CK19. a H69 cells were seeded into the platform, and the cells were observed under a light microscope over a 10-day period. Scale bar = 100 μm. Original magnification: ×40. b Data in the graph are presented as the average size of ~ 50 spheroids at each time point. Error bars are means ± SEM. P-values were obtained from Student’s t-test. ***P < 0.001 versus untreated controls. c Representative immunofluorescence images of Ki67 and CK19 in the spheroids at each time point. Spheroid cryosections were incubated with Ki67 or CK19 antibodies, followed by incubation with Alexa Fluor 488-conjugated secondary antibodies. Scale bar = 100 μm. Original magnification: ×40

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