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Fig. 6 | Parasites & Vectors

Fig. 6

From: Transcriptomic profiling of three-dimensional cholangiocyte spheroids long term exposed to repetitive Clonorchis sinensis excretory-secretory products

Fig. 6

Significantly regulated gene expressions of human intrahepatic cholangiocarcinoma (iCCA) identified from microarray and RNA-Seq of ESP-treated H69 spheroids. a Hierarchically clustered genes (rows) and experimental samples (columns) with dendrograms and clusters for iCCA RNA-Seq data (GSE 33327). RNA-Seq raw data were reanalyzed at the transcript level. Magenta in the heatmap indicates upregulation, while green denotes downregulation of gene expressions based on z-score. b Volcano plot illustrating genes from the filtered data set that are significant DEGs between normal and iCCA. c Venn diagram of DEGs from ESP-treated H69 spheroids using microarray and RNA-Seq. Gene expression of 69 overlapping genes between microarray and RNA-Seq was verified in 1864 significantly regulated genes from iCCA RNA-Seq data. d DEFB1, ID3, and LAD1 genes were identified as being significantly upregulated in iCCA, and RNASE4 was identified as a downregulated gene in iCCA compared to normal tissue-matched control. Error bars are means ± SEM. P values were obtained from Student’s t-test

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